The gallery opened in 1974 as an experimental satellite of the Norman Mackenzie Gallery. Two years later, members of the community formed the Rosemont Art Gallery Society Inc. to assume responsibility for its operation. In 1984, the Gallery entered into a partnership arrangement with the City of Regina to take over the gallery space in the Neil Balkwill Civic Arts Centre, a busy studio facility owned and operated by the City. The gallery occupies a 1,554 square foot exhibition space in the Centre along with an administrative area and storage facilities.

In May 2005, the gallery changed its name from the Rosemont Art Gallery to the Art Gallery of Regina.

The Art Gallery of Regina and the Neil Balkwill Civic Arts Cenre are located along Wascana Creek in Regina's Les Sherman Park. Access to the parking lot is available from 15th Avenue and McTavish Street. The facility is entirely wheelchair accessible.

The Art Gallery of Regina Inc. is an independent non-profit corporation with charitable status funded by the Regina Arts Commission, the Saskatchewan Arts Board, SaskCulture , Saskatchewan Lotteries Trust Fund for Sport, Culture and Recreation, the Canada Council for the Arts, and donations from individuals and corporate sponsors.